Labelling proteins for paramagnetic NMR Spectroscopy and EPR Spectroscopy.
Paramagnetic nuclear paramagnetic resonance (NMR) spectroscopy and Double Electron-Electron Resonance (DEER) experiments provide powerful tools to study the structures of biological macromolecules, especially proteins. This thesis focuses on two major objectives. (1) It demonstrates the application of these techniques to answer questions in structure biology. (2) A second focus is on the development of new spectroscopic labels and labelling strategies to improve the sensitivity, accuracy and...[Show more]
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