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Directed -in vitro- evolution of Precambrian and extant Rubiscos

Gomez-Fernandez, Bernardo; Garcia-Ruiz, Eva; Martin-Diaz, Javier; de Santos, Patricia Gomez; Santos-Moriano, Paloma; Plou, Francisco J.; Ballesteros, Antonio; Garcia, Monica; Rodriguez, Marisa; Risso, Valeria A.; Sanchez-Ruiz, Jose M.; Whitney, Spencer

Description

Rubisco is an ancient, catalytically conserved yet slow enzyme, which plays a central role in the biosphere’s carbon cycle. The design of Rubiscos to increase agricultural productivity has hitherto relied on the use of in vivo selection systems, precluding the exploration of biochemical traits that are not wired to cell survival. We present a directed -in vitro- evolution platform that extracts the enzyme from its biological context to provide a new avenue for Rubisco engineering. Precambrian...[Show more]

CollectionsANU Research Publications
Date published: 2018
Type: Journal article
URI: http://hdl.handle.net/1885/160533
Source: Scientific Reports
DOI: 10.1038/s41598-018-23869-3
Access Rights: Open Access

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