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Transgene silencing and transgene-derived siRNA production in tobacco plants homozygous for an introduced AtMYB90 construct

Velten, Jeff; Cakir, Cahid; Youn, Eunseog; Chen, Junping; Cazzonelli, Christopher I.

Description

Transgenic tobacco (Nicotiana tabacum) lines were engineered to ectopically over-express AtMYB90 (PAP2), an R2-R3 Myb gene associated with regulation of anthocyanin production in Arabidopsis thaliana. Independently transformed transgenic lines, Myb27 and Myb237, accumulated large quantities of anthocyanin, generating a dark purple phenotype in nearly all tissues. After self-fertilization, some progeny of the Myb27 line displayed an unexpected pigmentation pattern, with most leaves displaying...[Show more]

CollectionsANU Research Publications
Date published: 2012-02-17
Type: Journal article
URI: http://hdl.handle.net/1885/15999
Source: PLoS ONE
DOI: 10.1371/journal.pone.0030141

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