Diffusion of CO2 across the mesophyll-bundle sheath cell interface in a C4 plant with genetically reduced PEP carboxylase activity
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Alonso Cantabrana, Hugo; Cousins, Asaph B.; Danila, Florence; Ryan, Timothy; Sharwood, Robert; von Caemmerer, Susanne; Furbank, Robert
Description
Phosphoenolpyruvate carboxylase (PEPC), localized to the cytosol of the mesophyll cell, catalyzes the first carboxylation step of the C4 photosynthetic pathway. Here, we used RNA interference to target the cytosolic photosynthetic PEPC isoform in Setaria viridis and isolated independent transformants with very low PEPC activities. These plants required high ambient CO2 concentrations for growth, consistent with the essential role of PEPC in C4 photosynthesis. The combination of estimating...[Show more]
dc.contributor.author | Alonso Cantabrana, Hugo | |
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dc.contributor.author | Cousins, Asaph B. | |
dc.contributor.author | Danila, Florence | |
dc.contributor.author | Ryan, Timothy | |
dc.contributor.author | Sharwood, Robert | |
dc.contributor.author | von Caemmerer, Susanne | |
dc.contributor.author | Furbank, Robert | |
dc.date.accessioned | 2019-04-15T11:46:35Z | |
dc.identifier.issn | 0032-0889 | |
dc.identifier.uri | http://hdl.handle.net/1885/159675 | |
dc.description.abstract | Phosphoenolpyruvate carboxylase (PEPC), localized to the cytosol of the mesophyll cell, catalyzes the first carboxylation step of the C4 photosynthetic pathway. Here, we used RNA interference to target the cytosolic photosynthetic PEPC isoform in Setaria viridis and isolated independent transformants with very low PEPC activities. These plants required high ambient CO2 concentrations for growth, consistent with the essential role of PEPC in C4 photosynthesis. The combination of estimating direct CO2 fixation by the bundle sheath using gas-exchange measurements and modeling C4 photosynthesis with low PEPC activity allowed the calculation of bundle sheath conductance to CO2 diffusion (gbs) in the progeny of these plants. Measurements made at a range of temperatures suggested no or negligible effect of temperature on gbs depending on the technique used to calculate gbs. Anatomical measurements revealed that plants with reduced PEPC activity had reduced cell wall thickness and increased plasmodesmata (PD) density at the mesophyll-bundle sheath (M-BS) cell interface, whereas we observed little difference in these parameters at the mesophyll-mesophyll cell interface. The increased PD density at the M-BS interface was largely driven by an increase in the number of PD pit fields (cluster of PDs) rather than an increase in PD per pit field or the size of pit fields. The correlation of gbs with bundle sheath surface area per leaf area and PD area per M-BS area showed that these parameters and cell wall thickness are important determinants of gbs. It is intriguing to speculate that PD development is responsive to changes in C4 photosynthetic flux. | |
dc.description.sponsorship | This research was supported by the Bill and Melinda Gates Foundation’s funding for the C4 Rice consortium and by the Australian Research Council (ARC) Centre of Excellence for Translational Photosynthesis (CE140100015). R.E.S. is funded by ARC DECRA (DE130101760), and A.B.C. was supported by the Division of Chemical Sciences, Geosciences, and Biosciences, Office of Basic Energy Sciences, Photosynthetic Systems (DE-SC0001685) and the Edward R. Meyer Distinguished Professorship in Sciences. | |
dc.format.mimetype | application/pdf | |
dc.language.iso | en_AU | |
dc.publisher | American Society of Plant Biologists | |
dc.source | Plant Physiology | |
dc.title | Diffusion of CO2 across the mesophyll-bundle sheath cell interface in a C4 plant with genetically reduced PEP carboxylase activity | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.identifier.citationvolume | 178 | |
dc.date.issued | 2018 | |
local.identifier.absfor | 060705 - Plant Physiology | |
local.identifier.ariespublication | u4485658xPUB1328 | |
local.type.status | Published Version | |
local.contributor.affiliation | Alonso Cantabrana, Hugo, College of Science, ANU | |
local.contributor.affiliation | Cousins, Asaph B , Washington State University | |
local.contributor.affiliation | Danila , Florence, College of Science, ANU | |
local.contributor.affiliation | Ryan, Timothy, College of Science, ANU | |
local.contributor.affiliation | Sharwood, Robert, College of Science, ANU | |
local.contributor.affiliation | von Caemmerer, Susanne, College of Science, ANU | |
local.contributor.affiliation | Furbank, Robert, College of Science, ANU | |
dc.relation | http://purl.org/au-research/grants/arc/CE140100015 | |
dc.relation | http://purl.org/au-research/grants/arc/DE130101760 | |
local.bibliographicCitation.issue | 1 | |
local.identifier.doi | 10.1104/pp.18.00618 | |
local.identifier.absseo | 970106 - Expanding Knowledge in the Biological Sciences | |
dc.date.updated | 2019-03-12T07:28:42Z | |
local.identifier.scopusID | 2-s2.0-85054588579 | |
dcterms.accessRights | Open Access | |
dc.provenance | https://tpc.msubmit.net/cgi-bin/main.plex?form_type=display_auth_instructions...The journals of the American Society of Plant Biologists (ASPB), Plant Physiology and The Plant Cell, make all articles free 12 months after publication...Permission to reuse part or all of a copyrighted work published in an ASPB journal is granted without fee for personal or educational use, provided that copies are not made or distributed for profit or commercial advantage and that copies bear the full citation, the journal URL (www.plantphysiol.org; www.plantcell.org ), and the following notice: “Copyright American Society of Plant Biologists.” (as at 26/5/20) | |
Collections | ANU Research Publications |
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