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Diffusion of CO2 across the mesophyll-bundle sheath cell interface in a C4 plant with genetically reduced PEP carboxylase activity

Alonso Cantabrana, Hugo; Cousins, Asaph B.; Danila, Florence; Ryan, Timothy; Sharwood, Robert; von Caemmerer, Susanne; Furbank, Robert


Phosphoenolpyruvate carboxylase (PEPC), localized to the cytosol of the mesophyll cell, catalyzes the first carboxylation step of the C4 photosynthetic pathway. Here, we used RNA interference to target the cytosolic photosynthetic PEPC isoform in Setaria viridis and isolated independent transformants with very low PEPC activities. These plants required high ambient CO2 concentrations for growth, consistent with the essential role of PEPC in C4 photosynthesis. The combination of estimating...[Show more]

CollectionsANU Research Publications
Date published: 2018
Type: Journal article
Source: Plant Physiology
DOI: 10.1104/pp.18.00618
Access Rights: Open Access


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