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Conversion of an amide to a high-energy thioester by Staphylococcus aureus sortase A is powered by variable binding affinity for calcium

Wang, Xiao; Chen, Jia-Liang; Otting, Gottfried; Su, Xun-Cheng


Thioesters are key intermediates in biology, which often are generated from less energy-rich amide precursors. Staphylococcus aureus sortase A (SrtA) is an enzyme widely used in biotechnology for peptide ligation. The reaction proceeds in two steps, where the first step involves the conversion of an amide bond of substrate peptide into a thioester intermediate with the enzyme. Here we show that the free energy required for this step is matched by an about 30-fold increase in binding affinity of...[Show more]

CollectionsANU Research Publications
Date published: 2018
Type: Journal article
Source: Scientific Reports
DOI: 10.1038/s41598-018-34752-6
Access Rights: Open Access


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