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Fabrication of psoralen-loaded lipid-polymer hybrid nanoparticles and their reversal effect on drug resistance of cancer cells

Yuan, Yueling; Chiba, Peter; Cai, Tiange; Callaghan, Richard; Bai, Li; Cole, Susan P.C.; Cai, Yu

Description

In the present study, a lipid-polymer hybrid drug carrier system was developed to encapsulate psoralen (PSO), a multidrug resistance reversal agent and traditional Chinese medicine. Emphasis was focused the parameters that influence physicochemical characteristics, and then the drug release profile, stability, cytotoxicity and drug resistance reversal effect of the lipid-polymer hybrid nanoparticles (LPNs) were investigated. It was found that various formulation parameters affected NP size,...[Show more]

dc.contributor.authorYuan, Yueling
dc.contributor.authorChiba, Peter
dc.contributor.authorCai, Tiange
dc.contributor.authorCallaghan, Richard
dc.contributor.authorBai, Li
dc.contributor.authorCole, Susan P.C.
dc.contributor.authorCai, Yu
dc.date.accessioned2019-03-28T03:53:27Z
dc.date.available2019-03-28T03:53:27Z
dc.identifier.issn1021-335X
dc.identifier.urihttp://hdl.handle.net/1885/157363
dc.description.abstractIn the present study, a lipid-polymer hybrid drug carrier system was developed to encapsulate psoralen (PSO), a multidrug resistance reversal agent and traditional Chinese medicine. Emphasis was focused the parameters that influence physicochemical characteristics, and then the drug release profile, stability, cytotoxicity and drug resistance reversal effect of the lipid-polymer hybrid nanoparticles (LPNs) were investigated. It was found that various formulation parameters affected NP size, drug loading (DL) and release characteristics. Hydrophilic 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-carboxy(polyethylene glycol)2000 increased the ζ potential and thus the stability of the NPs, but also enlarged their diameter. The amount of PSO influenced their DL and encapsulation efficiency, but did not show any effect on drug release kinetics. Next, the stability of the LPNs in different media and their storage characteristics were assessed. Finally, the cytotoxicity and multidrug resistance reversal effect was studied in the K562 and HepG2 cell lines. The analysis of half maximal inhibitory concentration values demonstrated that combination therapy with doxorubicin (DOX) and PSO-loaded LPNs (P-LPNs) was 14- and 23-fold more effective than a single-dose DOX treatment in resistant K562 and HepG2 cells, respectively, and 2.2- and 2.1-fold more effective than a single-dose combination regimen of DOX and PSO in solution, respectively. These data indicate that the LPNs have superior properties compared with a combination therapy in solution.
dc.description.sponsorshipThis study was supported by the National Natural Science Foundation of China (grant no. 81273707), the Ministry of Education in the New Century Excellent Talents (grant no. NECT-12-0677), the Natural Science Foundation of Guangdong (grant nos. S2013010012880 and 2016A030311037), the Science and Technology Program of Guangzhou (grant nos. 2014J4500005 and 201704030141), the Science Program of the Department of Education of Guangdong (grant nos. 2013KJCX0021 and 2015KGJHZ012), the Science and Technology Program of Guangdong (grant no. 2015A050502027), and the Special Project of International Scientific and Technological Cooperation in Guangzhou Development District (grant no. 2017GH16).
dc.format.mimetypeapplication/pdf
dc.language.isoen_AU
dc.publisherSpandidos Publications
dc.rights© 2018
dc.sourceOncology Reports
dc.titleFabrication of psoralen-loaded lipid-polymer hybrid nanoparticles and their reversal effect on drug resistance of cancer cells
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume40
dc.date.issued2018
local.identifier.absfor111501 - Basic Pharmacology
local.identifier.ariespublicationa383154xPUB10384
local.publisher.urlhttps://www.spandidos-publications.com/
local.type.statusPublished Version
local.contributor.affiliationYuan, Yueling, Jinan University
local.contributor.affiliationChiba, Peter, Medical University of Vienna
local.contributor.affiliationCai, Tiange, Liaoning University
local.contributor.affiliationCallaghan, Richard, College of Science, ANU
local.contributor.affiliationBai, Li, Guangzhou Jiayuan Pharmaceutical Technology Co
local.contributor.affiliationCole, Susan P.C., Queen's University
local.contributor.affiliationCai, Yu, Jinan University
local.bibliographicCitation.issue2
local.bibliographicCitation.startpage1
local.bibliographicCitation.lastpage9
local.identifier.doi10.3892/or.2018.6492
local.identifier.absseo970106 - Expanding Knowledge in the Biological Sciences
dc.date.updated2019-03-12T07:19:34Z
local.identifier.scopusID2-s2.0-85048942891
dcterms.accessRightsOpen Access
dc.provenancehttp://sherpa.mimas.ac.uk/romeo/issn/1021-335X/..."author can archive publisher's version/PDF. 6 months embargo" from SHERPA/RoMEO site (as at 28/03/19).
CollectionsANU Research Publications

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