Skip navigation
Skip navigation
Open Research will be down for maintenance between 8:00 and 8:15 am on Tuesday, December 1 2020.

Two Histidines in an α‐Helix: A Rigid Co2+‐Binding Motif for PCS Measurements by NMR Spectroscopy

Bahramzadeh, Alireza; Jiang, Hailun; Huber, Thomas; Otting, Gottfried


Pseudocontact shifts (PCS) generated by paramagnetic metal ions present valuable long‐range information in the study of protein structural biology by nuclear magnetic resonance (NMR) spectroscopy. Faithful interpretation of PCSs, however, requires complete immobilization of the metal ion relative to the protein, which is difficult to achieve with synthetic metal tags. We show that two histidine residues in sequential turns of an α‐helix provide a binding site for a Co2+ ion, which positions the...[Show more]

CollectionsANU Research Publications
Date published: 2018-04-26
Type: Journal article
Source: Angewandte Chemie International Edition
DOI: 10.1002/anie.201802501
Access Rights: Open Access


File Description SizeFormat Image
manuscript.pdf839.49 kBAdobe PDFThumbnail
supporting information.pdf2.6 MBAdobe PDFThumbnail

Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.

Updated:  19 May 2020/ Responsible Officer:  University Librarian/ Page Contact:  Library Systems & Web Coordinator