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Two Histidines in an α‐Helix: A Rigid Co2+‐Binding Motif for PCS Measurements by NMR Spectroscopy

Bahramzadeh, Alireza; Jiang, Hailun; Huber, Thomas; Otting, Gottfried

Description

Pseudocontact shifts (PCS) generated by paramagnetic metal ions present valuable long‐range information in the study of protein structural biology by nuclear magnetic resonance (NMR) spectroscopy. Faithful interpretation of PCSs, however, requires complete immobilization of the metal ion relative to the protein, which is difficult to achieve with synthetic metal tags. We show that two histidine residues in sequential turns of an α‐helix provide a binding site for a Co2+ ion, which positions the...[Show more]

CollectionsANU Research Publications
Date published: 2018-04-26
Type: Journal article
URI: http://hdl.handle.net/1885/157091
Source: Angewandte Chemie International Edition
DOI: 10.1002/anie.201802501
Access Rights: Open Access

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