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Unlocking HDR-mediated nucleotide editing by identifying high-efficiency target sites using machine learning

O’Brien, Aidan R.; Wilson, Laurence O. W.; Burgio, Gaetan; Bauer, Denis C.


Editing individual nucleotides is a crucial component for validating genomic disease association. It is currently hampered by CRISPR-Cas-mediated “base editing” being limited to certain nucleotide changes, and only achievable within a small window around CRISPR-Cas target sites. The more versatile alternative, HDR (homology directed repair), has a 3-fold lower efciency with known optimization factors being largely immutable in experiments. Here, we investigated the variable...[Show more]

CollectionsANU Research Publications
Date published: 2019-02-26
Type: Journal article
Source: Scientific Reports
DOI: 10.1038/s41598-019-39142-0
Access Rights: Open access via publisher website
Open access via publisher website


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