Systems-guided forward genetic screen reveals a critical role of the replication stress response protein ETAA1 in T cell clonal expansion
Miosge, Lisa A.; Sontani, Yovina; Chuah, Aaron; Horikawa, Keisuke; Russell, Tiffany A; Mei, Yan; Wagle, Mayura V; Howard, Debbie; Enders, Anselm; Tscharke, David C; Goodnow, Christopher C; Parish, Ian A
T-cell immunity requires extremely rapid clonal proliferation of rare, antigen-specific T lymphocytes to form effector cells. Here we identify a critical role for ETAA1 in this process by surveying random germ line mutations in mice using exome sequencing and bioinformatic annotation to prioritize mutations in genes of unknown function with potential effects on the immune system, followed by breeding to homozygosity and testing for immune system phenotypes. Effector CD8+ and CD4+ T-cell...[Show more]
|Collections||ANU Research Publications|
|Source:||Proceedings of the National Academy of Sciences|
|Access Rights:||Open Access|
|Proc Natl Acad Sci USA 2017 Miosge.pdf||Journal article||1.14 MB||Adobe PDF|
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