The Escherichia coli glucuronylsynthase promoted synthesis of steroid glucuronides: improved practicality and broader scope
A library of steroid glucuronides was prepared using the glucuronylsynthase derived from Escherichia coliβ-glucuronidase, followed by purification using solid-phase extraction. A representative range of steroid substrates were screened for synthesis on the milligram scale under optimised conditions with conversions dependent on steroid substitution and stereochemistry. Epiandrosterone (3β-hydroxy-5α-androstan-17-one) provided the highest conversion of 90% (84% isolated yield). The previously...[Show more]
|Collections||ANU Research Publications|
|Source:||Organic & biomolecular chemistry|
|Ma et al The Escherichia Coli 2014.pdf||792.77 kB||Adobe PDF|
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