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The Escherichia coli glucuronylsynthase promoted synthesis of steroid glucuronides: improved practicality and broader scope

Ma, Paul; Kanizaj, Nicholas; Chan, Shu-Ann; Ollis, David L; McLeod, Malcolm D.

Description

A library of steroid glucuronides was prepared using the glucuronylsynthase derived from Escherichia coliβ-glucuronidase, followed by purification using solid-phase extraction. A representative range of steroid substrates were screened for synthesis on the milligram scale under optimised conditions with conversions dependent on steroid substitution and stereochemistry. Epiandrosterone (3β-hydroxy-5α-androstan-17-one) provided the highest conversion of 90% (84% isolated yield). The previously...[Show more]

CollectionsANU Research Publications
Date published: 2014-07-01
Type: Journal article
URI: http://hdl.handle.net/1885/15428
Source: Organic & biomolecular chemistry
DOI: 10.1039/c4ob00984c

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