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Pseudomonas aeruginosa arylsulfatase: a purified enzyme for the mild hydrolysis of steroid sulfates

Stevenson, Bradley J.; Waller, Christopher C.; Ma, Paul; Li, Kunkun; Cawley, Adam T.; Ollis, David L.; McLeod, Malcolm D.


The hydrolysis of sulfate ester conjugates is frequently required prior to analysis for a range of analytical techniques including gas chromatography-mass spectrometry (GC-MS). Sulfate hydrolysis may be achieved with commercial crude arylsulfatase enzyme preparations such as that derived from Helix pomatia but these contain additional enzyme activities such as glucuronidase, oxidase, and reductase that make them unsuitable for many analytical applications. Strong acid can also be used to...[Show more]

CollectionsANU Research Publications
Date published: 2015
Type: Journal article
Source: Drug Testing and Analysis
DOI: 10.1002/dta.1782


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