PScan 1.0: flexible software framework for polygon based multiphoton microscopy
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Li, Yongxiao; Lee, Woei Ming (Steve)
Description
Multiphoton laser scanning microscopes exhibit highly localized nonlinear optical excitation and are powerful instruments for in-vivo deep tissue imaging. Customized multiphoton microscopy has a significantly superior performance for in-vivo imaging because of precise control over the scanning and detection system. To date, there have been several flexible software platforms catered to custom built microscopy systems i.e. ScanImage, HelioScan, MicroManager, that perform at imaging speeds of...[Show more]
dc.contributor.author | Li, Yongxiao | |
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dc.contributor.author | Lee, Woei Ming (Steve) | |
dc.coverage.spatial | Adelaide, Australia | |
dc.date.accessioned | 2018-11-30T01:19:52Z | |
dc.date.available | 2018-11-30T01:19:52Z | |
dc.date.created | October 17-19 2016 | |
dc.identifier.isbn | 9781510604346 | |
dc.identifier.uri | http://hdl.handle.net/1885/154217 | |
dc.description.abstract | Multiphoton laser scanning microscopes exhibit highly localized nonlinear optical excitation and are powerful instruments for in-vivo deep tissue imaging. Customized multiphoton microscopy has a significantly superior performance for in-vivo imaging because of precise control over the scanning and detection system. To date, there have been several flexible software platforms catered to custom built microscopy systems i.e. ScanImage, HelioScan, MicroManager, that perform at imaging speeds of 30-100fps. In this paper, we describe a flexible software framework for high speed imaging systems capable of operating from 5 fps to 1600 fps. The software is based on the MATLAB image processing toolbox. It has the capability to communicate directly with a high performing imaging card (Matrox Solios eA/XA), thus retaining high speed acquisition. The program is also designed to communicate with LabVIEW and Fiji for instrument control and image processing. Pscan 1.0 can handle high imaging rates and contains sufficient flexibility for users to adapt to their high speed imaging systems. © (2016) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only. | |
dc.format.mimetype | application/pdf | |
dc.publisher | SPIE - The International Society for Optical Engineering | |
dc.relation.ispartofseries | SPIE Biophotonics Australasia | |
dc.source | Proceedings of SPIE, Conference Volume 10013 | |
dc.source.uri | https://spie.org/BAU/conferencedetails/biophotonics-australasia | |
dc.title | PScan 1.0: flexible software framework for polygon based multiphoton microscopy | |
dc.type | Conference paper | |
local.description.notes | Imported from ARIES | |
local.description.refereed | Yes | |
dc.date.issued | 2016 | |
local.identifier.absfor | 090606 - Photonics and Electro-Optical Engineering (excl. Communications) | |
local.identifier.absfor | 090303 - Biomedical Instrumentation | |
local.identifier.ariespublication | u5117155xPUB98 | |
local.type.status | Published Version | |
local.contributor.affiliation | Li, Yongxiao, College of Engineering and Computer Science, ANU | |
local.contributor.affiliation | Lee, Woei Ming (Steve), College of Engineering and Computer Science, ANU | |
local.identifier.doi | 10.1117/12.2242954 | |
local.identifier.absseo | 970110 - Expanding Knowledge in Technology | |
local.identifier.absseo | 970109 - Expanding Knowledge in Engineering | |
dc.date.updated | 2018-11-29T08:22:47Z | |
local.identifier.scopusID | 2-s2.0-85011391957 | |
local.identifier.thomsonID | 000394526200046 | |
dcterms.accessRights | Open Access | |
Collections | ANU Research Publications |
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File | Description | Size | Format | Image |
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01_Li_PScan_1.0%3A_flexible_software_2016.pdf | 643.32 kB | Adobe PDF |
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