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Increasing antigen presentation on HSV-1-infected cells increases lesion size but does not alter neural infection or latency

Russell, Tiffany A; Velusamy, Thilaga; Tseng, Yeu-Yang; Tscharke, David C

Description

CD8+ T cells have a role in the control of acute herpes simplex virus (HSV) infection and may also be important in the maintenance of latency. In this study we have explored the consequences of boosting the efficacy of CD8+ T cells against HSV by increasing the amount of an MHC I-presented epitope on the surface of infected cells. To do this we used HSVs engineered to express an extra copy of the immunodominant CD8+ T cell epitope in C57Bl/6 mice, namely gB498 (SSIEFARL). Despite greater...[Show more]

dc.contributor.authorRussell, Tiffany A
dc.contributor.authorVelusamy, Thilaga
dc.contributor.authorTseng, Yeu-Yang
dc.contributor.authorTscharke, David C
dc.date.accessioned2018-11-30T01:05:39Z
dc.date.available2018-11-30T01:05:39Z
dc.identifier.issn0022-1317
dc.identifier.urihttp://hdl.handle.net/1885/153878
dc.description.abstractCD8+ T cells have a role in the control of acute herpes simplex virus (HSV) infection and may also be important in the maintenance of latency. In this study we have explored the consequences of boosting the efficacy of CD8+ T cells against HSV by increasing the amount of an MHC I-presented epitope on the surface of infected cells. To do this we used HSVs engineered to express an extra copy of the immunodominant CD8+ T cell epitope in C57Bl/6 mice, namely gB498 (SSIEFARL). Despite greater presentation of gB498 on infected cells, CD8+ T cell responses to these viruses in mice were similar to those elicited by a control virus. Further, the expression of extra gB498 did not significantly alter the extent or stability of latency in our mouse model, and virus loads in skin and sensory ganglia of infected mice were not affected. Surprisingly, mice infected with these viruses developed significantly larger skin lesions than those infected with control viruses and notably, this phenotype was dependent on MHC haplotype. Therefore increasing the visibility of HSV-infected cells to CD8+ T cell attack did not impact neural infection or latency, but rather enhanced pathology in the skin.
dc.format.mimetypeapplication/pdf
dc.rights2018 The Authors
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceThe Journal of General Virology
dc.subjectcd8+ t cells
dc.subjecthsv
dc.subjectantigen presentation
dc.subjectherpes simplex virus
dc.subjectimmunopathology
dc.subjectlatency
dc.titleIncreasing antigen presentation on HSV-1-infected cells increases lesion size but does not alter neural infection or latency
dc.typeJournal article
local.identifier.citationvolume99
dc.date.issued2018-05
local.type.statusPublished Version
local.identifier.essn1465-2099
local.bibliographicCitation.issue5
local.bibliographicCitation.startpage682-692
local.bibliographicCitation.lastpage692
local.identifier.doi10.1099/jgv.0.001059
dcterms.accessRightsOpen Access
dc.rights.licenseThis is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
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