Rbf Regulates Drosophila Spermatogenesis via Control of Somatic Stem and Progenitor Cell Fate in the Larval Testis
-
Altmetric Citations
Quinn, Leonie; Dominado, Nicole; La Marca, John E.; Siddall, Nicole A.; Heaney, James; Tran, Mai; Cai, Yu; Yu, Fengwei; Wang, Hongyan; Somers, W. Gregory; Hime, Gary R.
Description
The Drosophila testis has been fundamental to understanding how stem cells interact with their endogenous microenvironment, or niche, to control organ growth in vivo. Here, we report the identification of two independent alleles for the highly conserved tumor suppressor gene, Retinoblastoma-family protein (Rbf), in a screen for testis phenotypes in X chromosome third-instar lethal alleles. Rbf mutant alleles exhibit overproliferation of spermatogonial cells, which is phenocopied by the...[Show more]
dc.contributor.author | Quinn, Leonie | |
---|---|---|
dc.contributor.author | Dominado, Nicole | |
dc.contributor.author | La Marca, John E. | |
dc.contributor.author | Siddall, Nicole A. | |
dc.contributor.author | Heaney, James | |
dc.contributor.author | Tran, Mai | |
dc.contributor.author | Cai, Yu | |
dc.contributor.author | Yu, Fengwei | |
dc.contributor.author | Wang, Hongyan | |
dc.contributor.author | Somers, W. Gregory | |
dc.contributor.author | Hime, Gary R. | |
dc.date.accessioned | 2018-11-29T22:57:20Z | |
dc.date.available | 2018-11-29T22:57:20Z | |
dc.identifier.issn | 2213-6711 | |
dc.identifier.uri | http://hdl.handle.net/1885/153834 | |
dc.description.abstract | The Drosophila testis has been fundamental to understanding how stem cells interact with their endogenous microenvironment, or niche, to control organ growth in vivo. Here, we report the identification of two independent alleles for the highly conserved tumor suppressor gene, Retinoblastoma-family protein (Rbf), in a screen for testis phenotypes in X chromosome third-instar lethal alleles. Rbf mutant alleles exhibit overproliferation of spermatogonial cells, which is phenocopied by the molecularly characterized Rbf11 null allele. We demonstrate that Rbf promotes cell-cycle exit and differentiation of the somatic and germline stem cells of the testes. Intriguingly, depletion of Rbf specifically in the germline does not disrupt stem cell differentiation, rather Rbf loss of function in the somatic lineage drives overproliferation and differentiation defects in both lineages. Together our observations suggest that Rbf in the somatic lineage controls germline stem cell renewal and differentiation non-autonomously via essential roles in the microenvironment of the germline lineage. | |
dc.format.mimetype | application/pdf | |
dc.publisher | Elsevier Science & Technology | |
dc.source | Stem Cell Reports | |
dc.title | Rbf Regulates Drosophila Spermatogenesis via Control of Somatic Stem and Progenitor Cell Fate in the Larval Testis | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.identifier.citationvolume | 7 | |
dc.date.issued | 2016 | |
local.identifier.absfor | 119999 - Medical and Health Sciences not elsewhere classified | |
local.identifier.ariespublication | u5192707xPUB4 | |
local.type.status | Published Version | |
local.contributor.affiliation | Quinn, Leonie, College of Health and Medicine, ANU | |
local.contributor.affiliation | Dominado, Nicole, The University of Melbourne | |
local.contributor.affiliation | La Marca, John E., La Trobe University | |
local.contributor.affiliation | Siddall, Nicole A., The University of Melbourne | |
local.contributor.affiliation | Heaney, James, University of Melbourne | |
local.contributor.affiliation | Tran, Mai, The University of Melbourne | |
local.contributor.affiliation | Cai, Yu, National University of Singapore | |
local.contributor.affiliation | Yu, Fengwei, National University of Singapore | |
local.contributor.affiliation | Wang, Hongyan, Duke-National University of Singapore | |
local.contributor.affiliation | Somers, W. Gregory, La Trobe University | |
local.contributor.affiliation | Hime, Gary R., The University of Melbourne | |
local.bibliographicCitation.issue | 6 | |
local.bibliographicCitation.startpage | 1152 | |
local.bibliographicCitation.lastpage | 1163 | |
local.identifier.doi | 10.1016/j.stemcr.2016.11.007 | |
dc.date.updated | 2018-11-29T08:17:22Z | |
local.identifier.scopusID | 2-s2.0-85004191366 | |
local.identifier.thomsonID | 000393188500012 | |
dcterms.accessRights | Open Access | |
Collections | ANU Research Publications |
Download
File | Description | Size | Format | Image |
---|---|---|---|---|
01_Quinn_Rbf_Regulates_Drosophila_2016.pdf | 7.04 MB | Adobe PDF |
Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.
Updated: 17 November 2022/ Responsible Officer: University Librarian/ Page Contact: Library Systems & Web Coordinator