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Rbf Regulates Drosophila Spermatogenesis via Control of Somatic Stem and Progenitor Cell Fate in the Larval Testis

Quinn, Leonie; Dominado, Nicole; La Marca, John E.; Siddall, Nicole A.; Heaney, James; Tran, Mai; Cai, Yu; Yu, Fengwei; Wang, Hongyan; Somers, W. Gregory; Hime, Gary R.

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The Drosophila testis has been fundamental to understanding how stem cells interact with their endogenous microenvironment, or niche, to control organ growth in vivo. Here, we report the identification of two independent alleles for the highly conserved tumor suppressor gene, Retinoblastoma-family protein (Rbf), in a screen for testis phenotypes in X chromosome third-instar lethal alleles. Rbf mutant alleles exhibit overproliferation of spermatogonial cells, which is phenocopied by the...[Show more]

dc.contributor.authorQuinn, Leonie
dc.contributor.authorDominado, Nicole
dc.contributor.authorLa Marca, John E.
dc.contributor.authorSiddall, Nicole A.
dc.contributor.authorHeaney, James
dc.contributor.authorTran, Mai
dc.contributor.authorCai, Yu
dc.contributor.authorYu, Fengwei
dc.contributor.authorWang, Hongyan
dc.contributor.authorSomers, W. Gregory
dc.contributor.authorHime, Gary R.
dc.date.accessioned2018-11-29T22:57:20Z
dc.date.available2018-11-29T22:57:20Z
dc.identifier.issn2213-6711
dc.identifier.urihttp://hdl.handle.net/1885/153834
dc.description.abstractThe Drosophila testis has been fundamental to understanding how stem cells interact with their endogenous microenvironment, or niche, to control organ growth in vivo. Here, we report the identification of two independent alleles for the highly conserved tumor suppressor gene, Retinoblastoma-family protein (Rbf), in a screen for testis phenotypes in X chromosome third-instar lethal alleles. Rbf mutant alleles exhibit overproliferation of spermatogonial cells, which is phenocopied by the molecularly characterized Rbf11 null allele. We demonstrate that Rbf promotes cell-cycle exit and differentiation of the somatic and germline stem cells of the testes. Intriguingly, depletion of Rbf specifically in the germline does not disrupt stem cell differentiation, rather Rbf loss of function in the somatic lineage drives overproliferation and differentiation defects in both lineages. Together our observations suggest that Rbf in the somatic lineage controls germline stem cell renewal and differentiation non-autonomously via essential roles in the microenvironment of the germline lineage.
dc.format.mimetypeapplication/pdf
dc.publisherElsevier Science & Technology
dc.sourceStem Cell Reports
dc.titleRbf Regulates Drosophila Spermatogenesis via Control of Somatic Stem and Progenitor Cell Fate in the Larval Testis
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume7
dc.date.issued2016
local.identifier.absfor119999 - Medical and Health Sciences not elsewhere classified
local.identifier.ariespublicationu5192707xPUB4
local.type.statusPublished Version
local.contributor.affiliationQuinn, Leonie, College of Health and Medicine, ANU
local.contributor.affiliationDominado, Nicole, The University of Melbourne
local.contributor.affiliationLa Marca, John E., La Trobe University
local.contributor.affiliationSiddall, Nicole A., The University of Melbourne
local.contributor.affiliationHeaney, James, University of Melbourne
local.contributor.affiliationTran, Mai, The University of Melbourne
local.contributor.affiliationCai, Yu, National University of Singapore
local.contributor.affiliationYu, Fengwei, National University of Singapore
local.contributor.affiliationWang, Hongyan, Duke-National University of Singapore
local.contributor.affiliationSomers, W. Gregory, La Trobe University
local.contributor.affiliationHime, Gary R., The University of Melbourne
local.bibliographicCitation.issue6
local.bibliographicCitation.startpage1152
local.bibliographicCitation.lastpage1163
local.identifier.doi10.1016/j.stemcr.2016.11.007
dc.date.updated2018-11-29T08:17:22Z
local.identifier.scopusID2-s2.0-85004191366
local.identifier.thomsonID000393188500012
dcterms.accessRightsOpen Access
CollectionsANU Research Publications

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