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Efficient multi-site two-photon functional imaging of neuronal circuits

Castanares, Michael; Gautam, Vini; Drury, Jack Duncan; Bachor, Hans; Daria, Vincent

Description

Two-photon imaging using high-speed multi-channel detectors is a promising approach for optical recording of cellular membrane dynamics at multiple sites. A main bottleneck of this technique is the limited number of photons captured within a short exposure time (~1ms). Here, we implement temporal gating to improve the two-photon fluorescence yield from holographically projected multiple foci whilst maintaining a biologically safe incident average power. We observed up to 6x improvement in the...[Show more]

CollectionsANU Research Publications
Date published: 2016
Type: Journal article
URI: http://hdl.handle.net/1885/153767
Source: Biomedical Optics Express
DOI: 10.1364/BOE.7.005325
Access Rights: Open Access

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