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Reverse evolution leads to genotypic incompatibility despite functional and active site convergence

Kaltenbach, Miriam; Jackson, Colin; Campbell, Eleeanor; Hollfelder, Florian; Tokuriki, Nobuhiko

Description

Understanding the extent to which enzyme evolution is reversible can shed light on the fundamental relationship between protein sequence, structure, and function. Here, we perform an experimental test of evolutionary reversibility using directed evolution from a phosphotriesterase to an arylesterase, and back, and examine the underlying molecular basis. We find that wild-type phosphotriesterase function could be restored (>104-fold activity increase), but via an alternative set of mutations....[Show more]

CollectionsANU Research Publications
Date published: 2015
Type: Journal article
URI: http://hdl.handle.net/1885/153713
Source: eLife
DOI: 10.7554/eLife.06492
Access Rights: Open Access

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