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Efficient backbone cyclization of linear peptides by a recombinant asparaginyl endopeptidase

Harris, Karen; Durek, Thomas; Kaas, Quentin; Poth, Aaron; Gilding, Edward; Conlan, Brendon; Saska, Ivana; Daly, Norelle; Van der Weerden, Nicole L.; Craik, David J; Anderson, Marilyn


Cyclotides are diverse plant backbone cyclized peptidesthat have attracted interest as pharmaceutical scaffolds, but fundamentals of their biosynthetic origin remain elusive. Backbone cyclization is a key enzyme-mediated step of cyclotide biosynthesis and confers a measure of stability on the resultant cyclotide. Furthermore, cyclization would be desirable for engineered peptides. Here we report the identification of four asparaginyl endopeptidases (AEPs), proteases implicated in cyclization,...[Show more]

CollectionsANU Research Publications
Date published: 2015
Type: Journal article
Source: Nature Communications
DOI: 10.1038/ncomms10199
Access Rights: Open Access


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