Efficient backbone cyclization of linear peptides by a recombinant asparaginyl endopeptidase
Cyclotides are diverse plant backbone cyclized peptidesthat have attracted interest as pharmaceutical scaffolds, but fundamentals of their biosynthetic origin remain elusive. Backbone cyclization is a key enzyme-mediated step of cyclotide biosynthesis and confers a measure of stability on the resultant cyclotide. Furthermore, cyclization would be desirable for engineered peptides. Here we report the identification of four asparaginyl endopeptidases (AEPs), proteases implicated in cyclization,...[Show more]
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