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Translational arrest due to cytoplasmic redox stress delays adaptation to growth on methanol and heterologous protein expression in a typical fed-batch culture of Pichia pastoris

Edwards-Jones, Bryn; Aw, Rochelle; Barton, Geraint R; Tredwell, Gregory; Bundy, Jacob G; Leak, David J


Results We have followed a typical fed-batch induction regime for heterologous protein production under the control of the AOX1 promoter using both microarray and metabolomic analysis. The genetic constructs involved 1 and 3 copies of the TRY1 gene, encoding human trypsinogen. In small-scale laboratory cultures, expression of the 3 copy-number construct induced the unfolded protein response (UPR) sufficiently that titres of extracellular trypsinogen were lower in the 3-copy construct than...[Show more]

CollectionsANU Research Publications
Date published: 2015
Type: Journal article
Source: PLOS ONE (Public Library of Science)
DOI: 10.1371/journal.pone.0119637
Access Rights: Open Access


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