Translational arrest due to cytoplasmic redox stress delays adaptation to growth on methanol and heterologous protein expression in a typical fed-batch culture of Pichia pastoris
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Edwards-Jones, Bryn; Aw, Rochelle; Barton, Geraint R; Tredwell, Gregory; Bundy, Jacob G; Leak, David J
Description
Results We have followed a typical fed-batch induction regime for heterologous protein production under the control of the AOX1 promoter using both microarray and metabolomic analysis. The genetic constructs involved 1 and 3 copies of the TRY1 gene, encoding human trypsinogen. In small-scale laboratory cultures, expression of the 3 copy-number construct induced the unfolded protein response (UPR) sufficiently that titres of extracellular trypsinogen were lower in the 3-copy construct than...[Show more]
Collections | ANU Research Publications |
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Date published: | 2015 |
Type: | Journal article |
URI: | http://hdl.handle.net/1885/153439 |
Source: | PLOS ONE (Public Library of Science) |
DOI: | 10.1371/journal.pone.0119637 |
Access Rights: | Open Access |
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File | Description | Size | Format | Image |
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01_Edwards-Jones_Translational_arrest_due_to_2015.pdf | 1.84 MB | Adobe PDF |
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