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A bioinformatic strategy for the detection, classification and analysis of bacterial autotransporters

Celik, Nermin; Webb, Chaille T.; Leyton, Denisse; Holt, Kathryn E; Heinz, Eva; Gorrell, Rebecca; Kwok, Terry; Naderer, Thomas; Strugnell, R; Speed, Terence P; Teasdale, Rohan D.; Likic, Vladimir A.; Lithgow, Trevor

Description

Autotransporters are secreted proteins that are assembled into the outer membrane of bacterial cells. The passenger domains of autotransporters are crucial for bacterial pathogenesis, with some remaining attached to the bacterial surface while others are released by proteolysis. An enigma remains as to whether autotransporters should be considered a class of secretion system, or simply a class of substrate with peculiar requirements for their secretion. We sought to establish a sensitive search...[Show more]

dc.contributor.authorCelik, Nermin
dc.contributor.authorWebb, Chaille T.
dc.contributor.authorLeyton, Denisse
dc.contributor.authorHolt, Kathryn E
dc.contributor.authorHeinz, Eva
dc.contributor.authorGorrell, Rebecca
dc.contributor.authorKwok, Terry
dc.contributor.authorNaderer, Thomas
dc.contributor.authorStrugnell, R
dc.contributor.authorSpeed, Terence P
dc.contributor.authorTeasdale, Rohan D.
dc.contributor.authorLikic, Vladimir A.
dc.contributor.authorLithgow, Trevor
dc.date.accessioned2018-11-29T22:56:03Z
dc.date.available2018-11-29T22:56:03Z
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/1885/153382
dc.description.abstractAutotransporters are secreted proteins that are assembled into the outer membrane of bacterial cells. The passenger domains of autotransporters are crucial for bacterial pathogenesis, with some remaining attached to the bacterial surface while others are released by proteolysis. An enigma remains as to whether autotransporters should be considered a class of secretion system, or simply a class of substrate with peculiar requirements for their secretion. We sought to establish a sensitive search protocol that could identify and characterize diverse autotransporters from bacterial genome sequence data. The new sequence analysis pipeline identified more than 1500 autotransporter sequences from diverse bacteria, including numerous species of Chlamydiales and Fusobacteria as well as all classes of Proteobacteria. Interrogation of the proteins revealed that there are numerous classes of passenger domains beyond the known proteases, adhesins and esterases. In addition the barrel-domain-a characteristic feature of autotransporters-was found to be composed from seven conserved sequence segments that can be arranged in multiple ways in the tertiary structure of the assembled autotransporter. One of these conserved motifs overlays the targeting information required for autotransporters to reach the outer membrane. Another conserved and diagnostic motif maps to the linker region between the passenger domain and barrel-domain, indicating it as an important feature in the assembly of autotransporters.
dc.format.mimetypeapplication/pdf
dc.publisherPublic Library of Science
dc.sourcePLOS ONE (Public Library of Science)
dc.subjectKeywords: autotransporter; bacterial protein; unclassified drug; amino acid sequence; article; bacterial genome; bacterial secretion system; bioinformatics; carboxy terminal sequence; Chlamydiaceae; crystal structure; Fusobacteria; nonhuman; nucleotide sequence; ph
dc.titleA bioinformatic strategy for the detection, classification and analysis of bacterial autotransporters
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume7
dc.date.issued2012
local.identifier.absfor060501 - Bacteriology
local.identifier.absfor060102 - Bioinformatics
local.identifier.absfor060110 - Receptors and Membrane Biology
local.identifier.ariespublicationa383154xPUB1529
local.type.statusPublished Version
local.contributor.affiliationCelik, Nermin, Monash University
local.contributor.affiliationWebb, Chaille T., Monash University
local.contributor.affiliationLeyton, Denisse, College of Science, ANU
local.contributor.affiliationHolt, Kathryn E, The University of Melbourne
local.contributor.affiliationHeinz, Eva, Monash University
local.contributor.affiliationGorrell, Rebecca, Monash University
local.contributor.affiliationKwok, Terry, Monash University
local.contributor.affiliationNaderer, Thomas, Monash University
local.contributor.affiliationStrugnell, R, University of Melbourne
local.contributor.affiliationSpeed, Terence P, Walter and Eliza Hall Institute of Medical Research
local.contributor.affiliationTeasdale, Rohan D., The University of Queensland
local.contributor.affiliationLikic, Vladimir A., The University of Melbourne
local.contributor.affiliationLithgow, Trevor, Monash University
local.bibliographicCitation.issue8
local.bibliographicCitation.startpage1
local.bibliographicCitation.lastpage15
local.identifier.doi10.1371/journal.pone.0043245
local.identifier.absseo970106 - Expanding Knowledge in the Biological Sciences
dc.date.updated2018-11-29T08:09:45Z
local.identifier.scopusID2-s2.0-84865048949
local.identifier.thomsonID000307500800064
dcterms.accessRightsOpen Access
CollectionsANU Research Publications

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