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A FAST AND EFFICIENT METHOD FOR PREPARATION OF HIGH QUALITY RNA FROM FUNGAL MYCELIA

Schumann, Ulrike; Smith, Neil A; Wang, Ming-Bo

Description

Background: Fungal RNA samples are usually isolated from fungal mycelia grown in liquid culture, which relies on prolific growth of the fungus in liquid media. The fungal biomass is then collected by vacuum filtration, which can result in low recovery for samples with reduced biomass due to poor growth in liquid media. Findings: Here we report an alternative culturing method, based on growth on solid media which is independent of the ability of a fungus to grow in liquid culture. We show that...[Show more]

CollectionsANU Research Publications
Date published: 2013
Type: Journal article
URI: http://hdl.handle.net/1885/153335
Source: BMC Research Notes
DOI: 10.1186/1756-0500-6-71
Access Rights: Open Access

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