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A generalised module for the selective extracellular accumulation of recombinant proteins

Sevastsyanovich, Yanina R; Leyton, Denisse; Wells, Timothy J; Wardius, Catherine A; Tveen-Jensen, Karina; Morris, Faye C; Knowles, Timothy J; Cunningham, Adam F; Cole, Jeffrey A; Henderson, Ian R

Description

Background: It is widely believed that laboratory strains of Escherichia coli, including those used for industrial production of proteins, do not secrete proteins to the extracellular milieu.Results: Here, we report the development of a generalised module, based on an E. coli autotransporter secretion system, for the production of extracellular recombinant proteins. We demonstrate that a wide variety of structurally diverse proteins can be secreted as soluble proteins when linked to the...[Show more]

CollectionsANU Research Publications
Date published: 2012
Type: Journal article
URI: http://hdl.handle.net/1885/153097
Source: Microbial Cell Factories
DOI: 10.1186/1475-2859-11-69
Access Rights: Open Access

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