Post-conversion targeted capture of modified cytosines in mammalian and plant genomes
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Li, Qing; Suzuki, Masako; Wendt, Jennifer; Patterson, Nicole; Eichten, Steven; Hermanson, Peter J; Green, Dawn; Jeddeloh, Jeffrey A.; Richmond, Todd A; Rosenbaum, Heidi; Burgess, Daniel L; Springer, Nathan M; Greally, John M
Description
We present a capture-based approach for bisulfiteconverted DNA that allows interrogation of predefined genomic locations, allowing quantitative and qualitative assessments of 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) at CG dinucleotides and in non-CG contexts (CHG, CHH) in mammalian and plant genomes. We show the technique works robustly and reproducibly using as little as 500 ng of starting DNA, with results correlating well with whole genome bisulfite sequencing data, and...[Show more]
Collections | ANU Research Publications |
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Date published: | 2015 |
Type: | Journal article |
URI: | http://hdl.handle.net/1885/152741 |
Source: | Nucleic Acids Research |
DOI: | 10.1093/nar/gkv244 |
Access Rights: | Open Access |
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01_Li_Post-conversion_targeted_2015.pdf | 4.1 MB | Adobe PDF |
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