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Experimental evidence for a hydride transfer mechanism in plant glycolate oxidase catalysis

Dellero, Younes; Mauve , Caroline; Boex-Fontvieille, Edouard; Flesch, Valerie; Jossier, Mathieu; Tcherkez, Guillaume; Hodges, Michael

Description

In plants, glycolate oxidase is involved in the photorespiratory cycle, one of the major fluxes at the global scale. To clarify both the nature of the mechanism and possible differences in glycolate oxidase enzyme chemistry from C3 and C4 plant species, we analyzed kinetic parameters of purified recombinant C3 (Arabidopsis thaliana) and C4 (Zea mays) plant enzymes and compared isotope effects using natural and deuterated glycolate in either natural or deuterated solvent. The 12C/13C isotope...[Show more]

CollectionsANU Research Publications
Date published: 2015
Type: Journal article
URI: http://hdl.handle.net/1885/152305
Source: Journal of Biological Chemistry
DOI: 10.1074/jbc.M114.618629
Access Rights: Open Access

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