Identification and characterization of glycoproteins on the spore surface of Clostridium difficile
In this study, we identify a major spore surface protein, BclA, and provide evidence that this protein is glycosylated. Following extraction of the spore surface, solubilized proteins were separated by one-dimensional PAGE and stained with glycostain to reveal a reactive high-molecular-mass region of approximately 600 kDa. Tandem mass spectrometry analysis of in-gel digests showed this band to contain peptides corresponding to a putative exosporangial glycoprotein (BclA3) and identified a...[Show more]
|Collections||ANU Research Publications|
|Source:||Journal of Bacteriology|
|Access Rights:||Open Access|
|01_Strong_Identification_and_2014.pdf||1.8 MB||Adobe PDF|
Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.