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Identification and characterization of glycoproteins on the spore surface of Clostridium difficile

Strong, Philippa C.R.; Fulton, Kelly M.; Aubry, Annie; Foote, Simon; Twine, Susan M.; Logan, Susan M.


In this study, we identify a major spore surface protein, BclA, and provide evidence that this protein is glycosylated. Following extraction of the spore surface, solubilized proteins were separated by one-dimensional PAGE and stained with glycostain to reveal a reactive high-molecular-mass region of approximately 600 kDa. Tandem mass spectrometry analysis of in-gel digests showed this band to contain peptides corresponding to a putative exosporangial glycoprotein (BclA3) and identified a...[Show more]

CollectionsANU Research Publications
Date published: 2014
Type: Journal article
Source: Journal of Bacteriology
DOI: 10.1128/JB.01469-14
Access Rights: Open Access


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