Exploring novel in planta and in vitro approaches for bioengineering Rubisco
The CO2-fixing enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) represents the major point of carbon entry into the biosphere. Rubisco has a slow catalytic rate and poorly discriminates between substrate CO2 and O2. Recycling the undesired product of O2-fixation consumes energy and releases fixed CO2. To maintain adequate levels of carbon assimilation, higher plants invest 30% of leaf nitrogen into Rubisco and require larger stomatal apertures, increasing water loss via...[Show more]
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