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Evaluation of differential gene expression during behavioral development in the honeybee using microarrays and northern blots

Kucharski, Robert; Maleszka, Ryszard

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Background: The honeybee (Apis mellifera) has been used with great success in a variety of behavioral studies. The lack of genomic tools in this species has, however, hampered efforts to provide genome-based explanations for behavioral data. We have combined the power of DNA arrays and the availability of distinct behavioral stages in honeybees to explore the dynamics of gene expression during adult development in this insect. In addition, we used caffeine treatment, a procedure that...[Show more]

dc.contributor.authorKucharski, Robert
dc.contributor.authorMaleszka, Ryszard
dc.date.accessioned2015-09-03T05:26:03Z
dc.date.available2015-09-03T05:26:03Z
dc.identifier.issn14656906
dc.identifier.urihttp://hdl.handle.net/1885/15151
dc.description.abstractBackground: The honeybee (Apis mellifera) has been used with great success in a variety of behavioral studies. The lack of genomic tools in this species has, however, hampered efforts to provide genome-based explanations for behavioral data. We have combined the power of DNA arrays and the availability of distinct behavioral stages in honeybees to explore the dynamics of gene expression during adult development in this insect. In addition, we used caffeine treatment, a procedure that accelerates learning abilities in honeybees, to examine changes in gene expression underlying drug-induced behavioral modifications. Results: Spotted microarrays containing several thousand cDNAs were interrogated with RNAs extracted from newly emerged worker bees, experienced foragers and caffeine-treated bees. Thirty-six differentially expressed cDNAs were verified by northern blot hybridization and characterized in silico by sequencing and database searches. Experienced foragers overexpressed royal jelly proteins, a putative imaginal disc growth factor, a transcriptional regulator (Stck) and several enzymes, including -glucosidases, aminopeptidases and glucose dehydrogenase. Naive workers showed increased expression of members of the SPARC and lectin families, heat-shock cognate proteins and several proteins related to RNA translation and mitochondrial function. A number of novel genes overexpressed in both naive and experienced bees, and genes induced by caffeine, have also been identified. Conclusions: We have shown the usefulness of this transcriptome-based approach for gene discovery, in particular in the context of the efficacy of drug treatment, in a model organism in which routine genetic techniques cannot be applied easily.
dc.description.sponsorshipThis work was supported by Human Frontier Science Program grant RGO134/1999-B.
dc.publisherBioMed Central
dc.rights© Kucharski and Maleszka, licensee BioMed Central Ltd 2002
dc.sourceGenome Biology
dc.subjectdifferential gene expression
dc.subjectbehavioral development
dc.subjecthoneybee
dc.subjectmicroarrays
dc.subjectnorthern blots
dc.titleEvaluation of differential gene expression during behavioral development in the honeybee using microarrays and northern blots
dc.typeJournal article
local.identifier.citationvolume3
dc.date.issued2002
local.type.statusPublished Version
local.contributor.affiliationKucharski, Robert, Visual Sciences, Research School of Biological Sciences, The Australian National University
local.contributor.affiliationMaleszka, Ryszard, Visual Sciences, Research School of Biological Sciences, The Australian National University
local.bibliographicCitation.issue2
local.bibliographicCitation.startpageresearch0007.1
local.bibliographicCitation.lastpageresearch0007.9
local.identifier.doi10.1186/gb-2002-3-2-research0007
CollectionsANU Research Publications

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