Directed evolution of glycolytic enzymes : glyceraldehyde-3-phosphate dehydrogenases
Glyceraldehyde-3-phosphate dehydrogenase (Gapdh) performs a vital step in glycolysis. An in vitro fermentative system is being developed to convert glucose to ethanol. The system is not cofactor neutral, so will eventually stall through production of adenosine triphosphate (ATP). The Gapdh catalysed step can be modified by use of a non-phosphorylating variant (GapN) of the enzyme. GapN catalyses the Gapdh oxidation and the next step of the pathway without the production of ATP through...[Show more]
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