Investigation of mechanisms and sequence requirements in Arabidopsis miR159-mediated gene silencing
MicroRNAs (miRNAs) are critical regulators of gene expression in both animals and plants. Although rapid progress has been achieved in identifying endogenous miRNAs, and artificial miRNAs have been utilized extensively in gene silencing experiments, debate and doubt still linger over the general principles of miRNA target recognition and their mode(s) of action in plants. It is widely accepted that animal miRNAs, which generally have central mismatches to targets, act through mRNA decay and...[Show more]
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