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Genetic incorporation of unnatural amino acids into proteins

Ugwumba, Isaac Nnamdi

Description

The introduction of unnatural amino acids with novel properties into proteins is potentially a useful tool for understanding and manipulating their functions. The development of an in vitro method for the incorporation of a fluorescent amino acid L-(7-hydroxycoumarin-4-yl)ethylglycine (Hco), into proteins is described here. An S30 extract was produced from E. coli culture containing a plasmid that encodes an evolved aminoacyl-tRNA synthetase specific for Hco (CouRS) and a suppressor tRNA...[Show more]

CollectionsOpen Access Theses
Type: Thesis (PhD)
URI: http://hdl.handle.net/1885/151439

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