Genetic incorporation of unnatural amino acids into proteins
The introduction of unnatural amino acids with novel properties into proteins is potentially a useful tool for understanding and manipulating their functions. The development of an in vitro method for the incorporation of a fluorescent amino acid L-(7-hydroxycoumarin-4-yl)ethylglycine (Hco), into proteins is described here. An S30 extract was produced from E. coli culture containing a plasmid that encodes an evolved aminoacyl-tRNA synthetase specific for Hco (CouRS) and a suppressor tRNA...[Show more]
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