New methods for structural biology by NMR spectroscopy
Selective isotope labelling is a commonly used approach to improve the spectral resolution in NMR spectra. In the present thesis, a new method was developed to suppress the isotope scrambling and isotope dilution of selectively 15N-labelled proteins as a result of metabolic amino acid conversions. Chapter 2 demonstrates how the suppression of pyridoxal-5'-phosphate (PLP) dependent enzyme activity suppresses isotope scrambling. Broadband inactivation of the PLP enzymes present in an E. coli cell...[Show more]
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