Using novel approaches to understand development: gene targeting, protein labelling and the development of the accessory glands in Drosophila melanogaster
Three very different approaches to understanding developmental biology were taken in this project. The first was an attempt to investigate the subcellular localisation of the Drosophila RhoGEF Pebble (Pbl) in live cells in real time, with an aim to further elucidate the role of Pbl in EMT and mesoderm migration in the embryo. This method, tetracysteine labelling, makes use of a 6 aa tag and a fluorescent, cell permeable, biarsenical ligand. Pioneered in Roger Tsien's laboratory, it utilises the...[Show more]
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