Precise optical manipulation techniques for exploring neuronal function
This thesis presents two optical techniques for manipulating neurons in the analysis of their function, namely time-gated holographic two-photon photostimulation and femtosecond laser dendrotomy. At the start of this thesis, no illumination technique had been able to activate synapses along the three-dimensional (3D) dendritic arbour of a neuron using realistic spatio-temporal patterns of light that resemble physiological stimuli, a prerequisite to a systematic study of synaptic integration....[Show more]
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