A lab-on-a-chip droplet platform for protein expression
Droplet based microfluidics are promising new tools for biological and chemical assays. So far, droplet based microfluidics hasn't been reported for a complete directed evolution. Especially, there is no report on OpdA enzyme evolution based on a robust droplet based microfluidics. In this Thesis, a high throughput and high sensitivity microfluidic droplet platform is developed for in vitro protein expressions. The first model protein used in these studies is the green fluorescence protein...[Show more]
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