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3D structure determination of a protein in living cells using paramagnetic NMR spectroscopy

Pan, Bin-Bin; Yang, Feng; Ye, Yansheng; Wu, Qiong; Li, Conggang; Huber, Thomas; Su, Xun-Cheng


Determining the three-dimensional structure of a protein in living cells remains particularly challenging. We demonstrated that the integration of site-specific tagging proteins and GPS-Rosetta calculations provides a fast and effective way of determining the structures of proteins in living cells, and in principle the interactions and dynamics of protein-ligand complexes.

CollectionsANU Research Publications
Date published: 2016-08-11
Type: Journal article
Source: Chemical communications (Cambridge, England)
DOI: 10.1039/c6cc05490k
Access Rights: Open Access


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