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Site-specific tagging proteins with a rigid, small and stable transition metal chelator, 8-hydroxyquinoline, for paramagnetic NMR analysis

Yang, Yin; Huang, Feng; Huber, Thomas; Su, Xun-Cheng

Description

Design of a paramagnetic metal binding motif in a protein is a valuable way for understanding the function, dynamics and interactions of a protein by paramagnetic NMR spectroscopy. Several strategies have been proposed to site-specifically tag proteins with paramagnetic lanthanide ions. Here we report a simple approach of engineering a transition metal binding motif via site-specific labelling of a protein with 2-vinyl-8-hydroxyquinoline (2V-8HQ). The protein-2V-8HQ adduct forms a stable...[Show more]

CollectionsANU Research Publications
Date published: 2016-02
Type: Journal article
URI: http://hdl.handle.net/1885/149437
Source: Journal of biomolecular NMR
DOI: 10.1007/s10858-016-0011-7
Access Rights: Open Access

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