Site-specific tagging proteins with a rigid, small and stable transition metal chelator, 8-hydroxyquinoline, for paramagnetic NMR analysis
Design of a paramagnetic metal binding motif in a protein is a valuable way for understanding the function, dynamics and interactions of a protein by paramagnetic NMR spectroscopy. Several strategies have been proposed to site-specifically tag proteins with paramagnetic lanthanide ions. Here we report a simple approach of engineering a transition metal binding motif via site-specific labelling of a protein with 2-vinyl-8-hydroxyquinoline (2V-8HQ). The protein-2V-8HQ adduct forms a stable...[Show more]
|Collections||ANU Research Publications|
|Source:||Journal of biomolecular NMR|
|Access Rights:||Open Access|
|Yang_et_al.pdf||Author/s Accepted Manuscript (AAM) / Post-print||1.22 MB||Adobe PDF|
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