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Selective Distance Measurements Using Triple Spin Labeling with Gd³⁺, Mn²⁺, and a Nitroxide

Wu, Zuyan; Feintuch, Akiva; Collauto, Alberto; Adams, Luke A; Aurelio, Luigi; Graham, Bim; Otting, Gottfried; Goldfarb, Daniella


Distance measurements by pulse electron paramagnetic resonance techniques, such as double electron-electron resonance (DEER, also called PELDOR), have become an established tool to explore structural properties of biomacromolecules and their assemblies. In such measurements a pair of spin labels provides a single distance constraint. Here we show that by employing three different types of spin labels that differ in their spectroscopic and spin dynamics properties it is possible to extract three...[Show more]

CollectionsANU Research Publications
Date published: 2017-11-02
Type: Journal article
Source: The journal of physical chemistry letters
DOI: 10.1021/acs.jpclett.7b01739


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