Pulse EPR-enabled interpretation of scarce pseudocontact shifts induced by lanthanide binding tags
Pseudocontact shifts (PCS) induced by tags loaded with paramagnetic lanthanide ions provide powerful long-range structure information, provided the location of the metal ion relative to the target protein is known. Usually, the metal position is determined by fitting the magnetic susceptibility anisotropy (Δχ) tensor to the 3D structure of the protein in an 8-parameter fit, which requires a large set of PCSs to be reliable. In an alternative approach, we used multiple Gd(3+)-Gd(3+) distances...[Show more]
|Collections||ANU Research Publications|
|Source:||Journal of biomolecular NMR|
|Access Rights:||Open Access|
|JBiomolNMR2016_64_39.pdf||736.84 kB||Adobe PDF|
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