Structure restraints from heteronuclear pseudocontact shifts generated by lanthanide tags at two different sites
Pseudocontact shifts (PCS) encode long-range information on 3D structures of protein backbones and side-chains. The level of structural detail that can be obtained increases with the number of different sites tagged with a paramagnetic metal ion to generate PCSs. Here we show that PCSs from two different sites can suffice to determine the structure of polypeptide chains and their location and orientation relative to the magnetic susceptibility tensor χ, provided that PCSs are available for 1H...[Show more]
|Collections||ANU Research Publications|
|Source:||Journal of biomolecular NMR|
|Access Rights:||Open Access|
|JBiomolNMR2017_68_19.pdf||Author/s Accepted Manuscript (AAM) / Post-print||7.84 MB||Adobe PDF|
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