A New Gd³⁺ Spin Label for Gd³⁺-Gd³⁺ Distance Measurements in Proteins Produces Narrow Distance Distributions
Gd(3+) tags have been shown to be useful for performing distance measurements in biomolecules via the double electron-electron resonance (DEER) technique at Q- and W-band frequencies. We introduce a new cyclen-based Gd(3+) tag that exhibits a relatively narrow electron paramagnetic resonance (EPR) spectrum, affording high sensitivity, and which yields exceptionally narrow Gd(3+)-Gd(3+) distance distributions in doubly tagged proteins owing to a very short tether. Both the maxima and widths of...[Show more]
|Collections||ANU Research Publications|
|Source:||The journal of physical chemistry letters|
|JPhysChemLett2015_6_5016.pdf||635.21 kB||Adobe PDF|
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