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A New Gd³⁺ Spin Label for Gd³⁺-Gd³⁺ Distance Measurements in Proteins Produces Narrow Distance Distributions

Abdelkader, Elwy H; Lee, Michael D; Feintuch, Akiva; Cohen, Marie Ramirez; Swarbrick, James D; Otting, Gottfried; Graham, Bim; Goldfarb, Daniella


Gd(3+) tags have been shown to be useful for performing distance measurements in biomolecules via the double electron-electron resonance (DEER) technique at Q- and W-band frequencies. We introduce a new cyclen-based Gd(3+) tag that exhibits a relatively narrow electron paramagnetic resonance (EPR) spectrum, affording high sensitivity, and which yields exceptionally narrow Gd(3+)-Gd(3+) distance distributions in doubly tagged proteins owing to a very short tether. Both the maxima and widths of...[Show more]

CollectionsANU Research Publications
Date published: 2015-12-17
Type: Journal article
Source: The journal of physical chemistry letters
DOI: 10.1021/acs.jpclett.5b02451


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