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3D Structure Determination of an Unstable Transient Enzyme Intermediate by Paramagnetic NMR Spectroscopy

Chen, Jia-Liang; Wang, Xiao; Yang, Feng; Cao, Chan; Otting, Gottfried; Su, Xun-Cheng


Enzyme catalysis relies on conformational plasticity, but structural information on transient intermediates is difficult to obtain. We show that the three-dimensional (3D) structure of an unstable, low-abundance enzymatic intermediate can be determined by nuclear magnetic resonance (NMR) spectroscopy. The approach is demonstrated for Staphylococcus aureus sortase A (SrtA), which is an established drug target and biotechnological reagent. SrtA is a transpeptidase that converts an amide bond of a...[Show more]

CollectionsANU Research Publications
Date published: 2016
Type: Journal article
Source: Angewandte Chemie (International ed. in English)
DOI: 10.1002/anie.201606223


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