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New Lanthanide Tag for the Generation of Pseudocontact Shifts in DNA by Site-Specific Ligation to a Phosphorothioate Group

Wu, Zuyan; Lee, Michael D; Carruthers, Thomas J; Szabo, Monika; Dennis, Matthew L; Swarbrick, James D; Graham, Bim; Otting, Gottfried


Pseudocontact shifts (PCS) generated by paramagnetic lanthanides provide a rich source of long-range structural restraints that can readily be measured by nuclear magnetic resonance (NMR) spectroscopy. Many different lanthanide-binding tags have been designed for site-specific tagging of proteins, but established routes for tagging DNA with a single metal ion rely on difficult chemical synthesis. Here we present a simple and practical strategy for site-specific tagging of inexpensive...[Show more]

CollectionsANU Research Publications
Date published: 2017-05-09
Type: Journal article
Source: Bioconjugate chemistry
DOI: 10.1021/acs.bioconjchem.7b00202
Access Rights: Open Access


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