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Protein conformation by EPR spectroscopy using gadolinium tags clicked to genetically encoded p-azido-L-phenylalanine

Abdelkader, Elwy; Feintuch, A; Yao, X; Adams, L A; Aurelio, L; Graham, B; Goldfarb, D; Otting, Gottfried

Description

Quantitative cysteine-independent ligation of a Gd(3+) tag to genetically encoded p-azido-L-phenylalanine via Cu(I)-catalyzed click chemistry is shown to deliver an exceptionally powerful tool for Gd(3+)-Gd(3+) distance measurements by double electron-electron resonance (DEER) experiments, as the position of the Gd(3+) ion relative to the protein can be predicted with high accuracy.

CollectionsANU Research Publications
Date published: 2015-11-14
Type: Journal article
URI: http://hdl.handle.net/1885/148489
Source: Chemical communications (Cambridge, England)
DOI: 10.1039/c5cc07121f
Access Rights: Open Access

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