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Double-Arm Lanthanide Tags Deliver Narrow Gd³⁺ -Gd³⁺ Distance Distributions in Double Electron-Electron Resonance (DEER) Measurements

Welegedara, Adarshi P; Yang, Yin; Lee, Michael D; Swarbrick, James D; Graham, Bim; Goldfarb, Daniella; Otting, Gottfried; Huber, Thomas


Double-arm cyclen-based Gd3+ tags are shown to produce accurate nanometer scale Gd3+ -Gd3+ distance measurements in double electron-electron resonance (DEER) experiments by confining the space accessible to the metal ion. The results show excellent agreement with predictions both for the maximum and width of the measured distance distributions. For distance measurements in proteins, the tags can be attached to two cysteine residues located in positions i and i+4, or i and i+8, of an α-helix. In...[Show more]

CollectionsANU Research Publications
Date published: 2017-08-25
Type: Journal article
Source: Chemistry - A European Journal
DOI: 10.1002/chem.201702521
Access Rights: Open Access


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