Double-Arm Lanthanide Tags Deliver Narrow Gd³⁺ -Gd³⁺ Distance Distributions in Double Electron-Electron Resonance (DEER) Measurements
Double-arm cyclen-based Gd3+ tags are shown to produce accurate nanometer scale Gd3+ -Gd3+ distance measurements in double electron-electron resonance (DEER) experiments by confining the space accessible to the metal ion. The results show excellent agreement with predictions both for the maximum and width of the measured distance distributions. For distance measurements in proteins, the tags can be attached to two cysteine residues located in positions i and i+4, or i and i+8, of an α-helix. In...[Show more]
|Collections||ANU Research Publications|
|Source:||Chemistry - A European Journal|
|Access Rights:||Open Access|
|ChemEurJ2017_23_11694.pdf||4.32 MB||Adobe PDF|
Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.