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Isolation of an antimicrobial compound produced by bacteria associated with reef-building corals

Raina, Jean-Baptiste; Tapiolas, Dianne; Motti, Cherie A; Foret, Sylvain; Seemann, Torsten; Tebben, Jan; Willis, Bette L; Bourne, David G

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Bacterial communities associated with healthy corals produce antimicrobial compounds that inhibit the colonization and growth of invasive microbes and potential pathogens. To date, however, bacteria-derived antimicrobial molecules have not been identified in reef-building corals. Here, we report the isolation of an antimicrobial compound produced by Pseudovibrio sp. P12, a common and abundant coral-associated bacterium. This strain was capable of metabolizing dimethylsulfoniopropionate (DMSP),...[Show more]

dc.contributor.authorRaina, Jean-Baptiste
dc.contributor.authorTapiolas, Dianne
dc.contributor.authorMotti, Cherie A
dc.contributor.authorForet, Sylvain
dc.contributor.authorSeemann, Torsten
dc.contributor.authorTebben, Jan
dc.contributor.authorWillis, Bette L
dc.contributor.authorBourne, David G
dc.date.accessioned2018-09-17T01:21:53Z
dc.date.available2018-09-17T01:21:53Z
dc.identifier.urihttp://hdl.handle.net/1885/147599
dc.description.abstractBacterial communities associated with healthy corals produce antimicrobial compounds that inhibit the colonization and growth of invasive microbes and potential pathogens. To date, however, bacteria-derived antimicrobial molecules have not been identified in reef-building corals. Here, we report the isolation of an antimicrobial compound produced by Pseudovibrio sp. P12, a common and abundant coral-associated bacterium. This strain was capable of metabolizing dimethylsulfoniopropionate (DMSP), a sulfur molecule produced in high concentrations by reef-building corals and playing a role in structuring their bacterial communities. Bioassay-guided fractionation coupled with nuclear magnetic resonance (NMR) and mass spectrometry (MS), identified the antimicrobial as tropodithietic acid (TDA), a sulfur-containing compound likely derived from DMSP catabolism. TDA was produced in large quantities by Pseudovibrio sp., and prevented the growth of two previously identified coral pathogens, Vibrio coralliilyticus and V. owensii, at very low concentrations (0.5 μg/mL) in agar diffusion assays. Genome sequencing of Pseudovibrio sp. P12 identified gene homologs likely involved in the metabolism of DMSP and production of TDA. These results provide additional evidence for the integral role of DMSP in structuring coral-associated bacterial communities and underline the potential of these DMSP-metabolizing microbes to contribute to coral disease prevention.
dc.description.sponsorshipThis work was supported by the ARC Centre of Excellence for Coral Reef Studies, AIMS@JCU, James Cook University and the Australian Institute of Marine Science.
dc.format20 pages
dc.format.mimetypeapplication/pdf
dc.publisherPeerJ
dc.rights© Copyright 2016 Raina et al. Distributed under Creative Commons CC-BY 4.0
dc.sourcePeerJ
dc.subjectalphaproteobacteria
dc.subjectantimicrobial compounds
dc.subjectcoral-associated bacteria
dc.subjectdisease
dc.titleIsolation of an antimicrobial compound produced by bacteria associated with reef-building corals
dc.typeJournal article
local.identifier.citationvolume4
dcterms.dateAccepted2016-07-19
dc.date.issued2016-08-18
local.publisher.urlhttps://peerj.com/
local.type.statusPublished Version
local.contributor.affiliationForet, Sylvain, Division of Ecology and Evolution, CoS Research School of Biology, The Australian National University
local.identifier.essn2167-8359
local.bibliographicCitation.startpagee2275
local.identifier.doi10.7717/peerj.2275
dcterms.accessRightsOpen Access
CollectionsANU Research Publications

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