Skip navigation
Skip navigation

XA21-specific induction of stress-related genes following Xanthomonas infection of detached rice leaves

Thomas, Nicholas C; Schwessinger, Benjamin; Liu, Furong; Chen, Huamin; Wei, Tong; Nguyen, Yen P; Shaker, Isaac W F; Ronald, Pamela C

Description

The rice XA21 receptor kinase confers robust resistance to the bacterial pathogen Xanthomonas oryzaepv. oryzae (Xoo). We developed a detached leaf infection assay to quickly and reliably measure activation of the XA21-mediated immune response using genetic markers. We used RNA sequencing of elf18 treated EFR:XA21:GFP plants to identify candidate genes that could serve as markers for XA21 activation. From this analysis, we identified eight genes that are up-regulated in both in elf18 treated...[Show more]

dc.contributor.authorThomas, Nicholas C
dc.contributor.authorSchwessinger, Benjamin
dc.contributor.authorLiu, Furong
dc.contributor.authorChen, Huamin
dc.contributor.authorWei, Tong
dc.contributor.authorNguyen, Yen P
dc.contributor.authorShaker, Isaac W F
dc.contributor.authorRonald, Pamela C
dc.date.accessioned2018-08-29T04:42:04Z
dc.date.available2018-08-29T04:42:04Z
dc.identifier.urihttp://hdl.handle.net/1885/146711
dc.description.abstractThe rice XA21 receptor kinase confers robust resistance to the bacterial pathogen Xanthomonas oryzaepv. oryzae (Xoo). We developed a detached leaf infection assay to quickly and reliably measure activation of the XA21-mediated immune response using genetic markers. We used RNA sequencing of elf18 treated EFR:XA21:GFP plants to identify candidate genes that could serve as markers for XA21 activation. From this analysis, we identified eight genes that are up-regulated in both in elf18 treated EFR:XA21:GFP rice leaves and Xoo infected XA21 rice leaves. These results provide a rapid and reliable method to assess bacterial-rice interactions.
dc.description.sponsorshipThis work was part of the DOE Joint BioEnergy Institute (http://www.jbei.org) supported by the US Department of Energy, Office of Science, Office of Biological and Environmental Research, through contract DE-AC02-05CH11231 between Lawrence Berkeley National Laboratory and the US Department of Energy. This work was also supported by the NIH (GM59962). BS was supported by a Human Frontiers Science Program longterm postdoctoral fellowship (LT000674/2012) and a Discovery Early Career Award (DE150101897).
dc.format15 pages
dc.format.mimetypeapplication/pdf
dc.publisherPeerJ
dc.rights© Copyright 2016 Thomas et al. Distributed under Creative Commons CC-BY 4.0
dc.sourcePeerJ
dc.subjectEFR
dc.subjectRNAseq
dc.subjectrice
dc.subjectXA21
dc.subjectXanthomonas
dc.titleXA21-specific induction of stress-related genes following Xanthomonas infection of detached rice leaves
dc.typeJournal article
local.identifier.citationvolume4
dcterms.dateAccepted2016-08-14
dc.date.issued2016-09-28
local.publisher.urlhttps://peerj.com/
local.type.statusSubmitted Version
local.contributor.affiliationSchwessinger, Benjamin, Division of Plant Sciences, CoS Research School of Biology, The Australian National University
dc.relationhttp://purl.org/au-research/grants/arc/DE150101897
local.identifier.essn2167-8359
local.bibliographicCitation.startpagee2446
local.identifier.doi10.7717/peerj.2446
dcterms.accessRightsOpen Access
CollectionsANU Research Publications

Download

File Description SizeFormat Image
01 Thomas N C et al XA21 specific induction 2016.pdf1.27 MBAdobe PDFThumbnail


Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.

Updated:  22 January 2019/ Responsible Officer:  University Librarian/ Page Contact:  Library Systems & Web Coordinator