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Genotyping of whole genome amplified reduced representation libraries reveals a cryptic population of Culicoides brevitarsis in the Northern Territory, Australia

Onyango, Maria G; Aitken, Nicola C; Jack, Cameron; Chuah, Aaron; Oguya, James; Djikeng, Appolinaire; Kemp, Steve; Bellis, Glenn A; Nicholas, Adrian; Walker, Peter J; Duchemin, Jean-Bernard

Description

BACKGROUND The advent of genotyping by Next Generation Sequencing has enabled rapid discovery of thousands of single nucleotide polymorphism (SNP) markers and high throughput genotyping of large populations at an affordable cost. Genotyping by sequencing (GBS), a reduced representation library sequencing method, allows highly multiplexed sequencing of genomic subsets. This method has limitations for small organisms with low amounts of genomic DNA, such as the bluetongue virus (BTV) vectors,...[Show more]

CollectionsANU Research Publications
Date published: 2016-09-30
Type: Journal article
URI: http://hdl.handle.net/1885/146699
Source: BMC genomics
DOI: 10.1186/s12864-016-3124-1

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