Analysis of turbidity progress curves from protein aggregation reactions
To investigate the individual and combined effects of protein molecular chaperone abilities on the aggregation of proteins a vital first step is the development of a robust and simple assay for examining the aggregation of proteins. By far the most common in vitro method to monitor protein aggregation is the turbidity assay. All protein aggregates scatter light in the visible wavelength region since their size ranges from nanometer to micrometers. This characteristic combined with a lack of...[Show more]
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