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Non-destructive Phenotyping of Lettuce Plants in Early Stages of Development with Optical Sensors

Simko, Ivan; Hayes, Ryan J; Furbank, Robert T

Description

Rapid development of plants is important for the production of 'baby-leaf' lettuce that is harvested when plants reach the four- to eight-leaf stage of growth. However, environmental factors, such as high or low temperature, or elevated concentrations of salt, inhibit lettuce growth. Therefore, non-destructive evaluations of plants can provide valuable information to breeders and growers. The objective of the present study was to test the feasibility of using non-destructive phenotyping with...[Show more]

dc.contributor.authorSimko, Ivan
dc.contributor.authorHayes, Ryan J
dc.contributor.authorFurbank, Robert T
dc.date.accessioned2018-07-18T02:53:59Z
dc.date.available2018-07-18T02:53:59Z
dc.identifier.urihttp://hdl.handle.net/1885/145123
dc.description.abstractRapid development of plants is important for the production of 'baby-leaf' lettuce that is harvested when plants reach the four- to eight-leaf stage of growth. However, environmental factors, such as high or low temperature, or elevated concentrations of salt, inhibit lettuce growth. Therefore, non-destructive evaluations of plants can provide valuable information to breeders and growers. The objective of the present study was to test the feasibility of using non-destructive phenotyping with optical sensors for the evaluations of lettuce plants in early stages of development. We performed the series of experiments to determine if hyperspectral imaging and chlorophyll fluorescence imaging can determine phenotypic changes manifested on lettuce plants subjected to the extreme temperature and salinity stress treatments. Our results indicate that top view optical sensors alone can accurately determine plant size to approximately 7 g fresh weight. Hyperspectral imaging analysis was able to detect changes in the total chlorophyll (RCC) and anthocyanin (RAC) content, while chlorophyll fluorescence imaging revealed photoinhibition and reduction of plant growth caused by the extreme growing temperatures (3 and 39°C) and salinity (100 mM NaCl). Though no significant correlation was found between Fv/Fm and decrease in plant growth due to stress when comparisons were made across multiple accessions, our results indicate that lettuce plants have a high adaptability to both low (3°C) and high (39°C) temperatures, with no permanent damage to photosynthetic apparatus and fast recovery of plants after moving them to the optimal (21°C) temperature. We have also detected a strong relationship between visual rating of the green- and red-leaf color intensity and RCC and RAC, respectively. Differences in RAC among accessions suggest that the selection for intense red color may be easier to perform at somewhat lower than the optimal temperature. This study serves as a proof of concept that optical sensors can be successfully used as tools for breeders when evaluating young lettuce plants. Moreover, we were able to identify the locus for light green leaf color (qLG4), and position this locus on the molecular linkage map of lettuce, which shows that these techniques have sufficient resolution to be used in a genetic context in lettuce.
dc.description.sponsorshipIS acknowledges the receipt of a fellowship from the OECD Co-operative Research Programme: Biological Resource Management for Sustainable Agricultural Systems in 2013.
dc.format19 pages
dc.publisherFrontiers Media
dc.rights© 2016 Simko, Hayes and Furbank. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
dc.sourceFrontiers in plant science
dc.subjectelevated salinity
dc.subjectphotosynthesis
dc.subjectrelative anthocyanin content
dc.subjectrelative chlorophyll content
dc.subjectrelative growth rate
dc.subjecttemperature stress
dc.subjectvisual rating of color intensity
dc.titleNon-destructive Phenotyping of Lettuce Plants in Early Stages of Development with Optical Sensors
dc.typeJournal article
local.identifier.citationvolume7
dcterms.dateAccepted2016-12-14
dc.date.issued2016-12-27
local.publisher.urlhttps://www.frontiersin.org/
local.type.statusPublished Version
local.contributor.affiliationFurbank, Robert T., Division of Plant Sciences, CoS Research School of Biology, The Australian National University
local.identifier.essn1664-462X
local.bibliographicCitation.startpage1985
local.identifier.doi10.3389/fpls.2016.01985
dcterms.accessRightsOpen Access
CollectionsANU Research Publications

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