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The importance of low level QC for high sensitivity troponin assays

Hickman, Peter E.; Koerbin, Gus; Badrick, Tony; Oakman, Carmen; Potter, Julia M.

Description

BACKGROUND With the advent of the new high-sensitivity troponin assays, it is becoming critical to measure troponin accurately to low concentrations. To ensure assay performance is acceptable, appropriate QC must be run. METHODS In addition to the routine use of commercial QC material, we prepared pools of human QC material with low troponin concentrations close to the limit of quantitation, and ran these regularly on our laboratory analysers. RESULTS Over 3 years we found no drift or shift in...[Show more]

dc.contributor.authorHickman, Peter E.
dc.contributor.authorKoerbin, Gus
dc.contributor.authorBadrick, Tony
dc.contributor.authorOakman, Carmen
dc.contributor.authorPotter, Julia M.
dc.date.accessioned2018-05-24T03:45:11Z
dc.identifier.issn0009-9120
dc.identifier.urihttp://hdl.handle.net/1885/143589
dc.description.abstractBACKGROUND With the advent of the new high-sensitivity troponin assays, it is becoming critical to measure troponin accurately to low concentrations. To ensure assay performance is acceptable, appropriate QC must be run. METHODS In addition to the routine use of commercial QC material, we prepared pools of human QC material with low troponin concentrations close to the limit of quantitation, and ran these regularly on our laboratory analysers. RESULTS Over 3 years we found no drift or shift in our hs-cTnI assay. We found that only the very low concentration human QC material gave warning of precision problems with the hs-cTnI assay. At the time of the documented poor assay precision, the higher concentration QC material indicated satisfactory performance. CONCLUSIONS Choice of QC material with an appropriate concentration is important for any assay. For hs-cTn assays, it is of particular importance to use control material with a concentration near to the limit of quantitation.
dc.format.mimetypeapplication/pdf
dc.publisherElsevier
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceClinical biochemistry
dc.subjectlimit of quantitation
dc.subjectqc
dc.subjecttroponin
dc.titleThe importance of low level QC for high sensitivity troponin assays
dc.typeJournal article
dc.date.issued2018-05-08
local.publisher.urlhttps://www.elsevier.com/
local.type.statusAccepted Version
local.contributor.affiliationHickman, Peter E., The Medical School, The Australian National University
local.contributor.affiliationPotter, Julia M., The Medical School, The Australian National University
local.identifier.essn1873-2933
local.identifier.doi10.1016/j.clinbiochem.2018.05.007
dcterms.accessRightsOpen Access
dc.provenancehttp://www.sherpa.ac.uk/romeo/issn/0009-9120/..."Author's post-print on open access repository after an embargo period of 12 months" from SHERPA/RoMEO site (as at 24/05/18).
dc.rights.licenseCC-BY-NC-ND 4.0 license
CollectionsANU Research Publications

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